This is “Where Things Grow Back!”

We are currently involved in a Research Study on a special process of purifying bone marrow or BMA using a system designed by Emcyte Corporation. We are working with Bio Sciences Research Center at Harvard and offering a great opportunity for special pricing on treatment. Call our office for details.

GEcko pic lipo

Prolotherapy & Platelet Rich Plasma 

Years of experience and training in the field of regenerative injections are why you might choose these physicians as your doctors for these treatments. Platelet Rich Plasma Therapy or PRP is Prolotherapy using your own blood. Your blood is placed into a machine that looks like a record player. The platelet rich plasma is spun down and this solution is then injected into area’s that are damaged or arthritic.

Click on one of the button’s below to explore videos and information on how Gecko’s Regenerative therapies work on some specific conditions.

Gecko Joint & Spine is Sarasota’s Regenerative Clinic!


“We are commited to offering the finest and most advanced regenerative injection therapies to my patients in a warm, caring and of course most comfortable/ pain free way possible.”

About PRP therapy.

This is a video explaining PRP.

This video shows how ligament damage can often be the cause of chronic neck pain.

Watch this video on PRP regenerating Cartilage.

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How are the cells deployed into a patient?

How are the cells deployed into a patient?

How are the cells deployed?Depending on the type of treatment required, stem cells can be injected through veins, arteries, into spinal fluid, subcutaneously, or directly into joints or organs. All of these are considered minimally invasive methods of introducing the stem cells. Stem cells injected intravenously are known to “seek out and find” (see photo) areas of tissue damage and migrate to that location thus potentially providing regenerative healing. Intravenously injected stem cells have been shown to have the capability of crossing the “blood-brain barrier” to enter the central nervous system and they can be identified in the patient’s body many months after deployment. Note yellow arrow showing the stem cells concentrated in the patient’s hand where he had a Dupytren’s contracture (Dupuytren’s contracture is a hand deformity that causes the tissue beneath the surface of the hand to thicken and contract).

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Why does Gecko Joint and Spine use liposuction fat rather than bone marrow as a source of stem cells?

Why does Gecko use liposuction fat rather than bone marrow as a source of stem cells?

Bone marrow sampling yields approximately 5,000 – 60,000 cells that are then cultured over several days to perhaps a few million cells prior to deployment (injection into the patient). Recent advances in stem cell science have made it possible to obtain high numbers of quality multi-potent (able to form numerous other tissues) cells from a person’s own liposuction fat. Gecko uses new technology to process this fat to yield approximately five hundred thousand to one million stem cells per cc of fat, and therefore, it is possible to obtain as many as 10 to 40 million cells from a single treatment. These adipose derived stem cells can form many different types of cells when deployed properly including bone, cartilage, tendon (connective tissue), muscle, blood vessels, nerve tissue and others. We also often with add the bone marrow stem cells into the injection which we call Marrapose. There are signaling compounds in Marrow that help the regenerative process along a bit seeing the shear volume of stem cells from the adipose bolsters the healing.

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Chiropractor Gets PRP injections for Chronic Back Pain from Arthritis

Screen Shot 2015-03-29 at 1.13.04 PMEven Chiropractors can’t escape the fact that adjustments won’t regenerate damaged connective tissue in the spine and pelvis (SI joints). This connective tissue is the stuff that hold everything together and when it’s damaged it leads to instability which then ends in painful arthritis. After years of pain and disability Doc gets Platelet Rich Plasma Prolotherapy at Gecko Joint and Spine in Sarasota Florida Chiropractor Gets PRP injections for Chronic Back Pain from Arthritis.

Free consult by calling (941) 330-8553

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PRP & ozone in Sarasota for arthritis of knee & hip under ultrasound guidance

This was a patient in Sarasota Florida with hip and knee arthritis that received platelet rich plasma with honey matrix and ozone or prolozone. These amazing regenerative injections where done under ultrasound guidance. Watch her post ultrasound scan and the improvement that was seen after just one treatment!

Call (941) 330-8553 for a free in office or phone consultation with on of our doctors.

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Stem Cells Treatment for Ankylosing Spondylitis in Sarasota Florida

Stem Cells Treatment for Ankylosing Spondylitis

Ankylosing Spondylitis is a form of spondyloarthritis, a chronic, inflammatory arthritis and autoimmune disease. It mainly affects joints in the spine and the sacroiliac joint in the pelvis, and can cause eventual fusion of the spine.

Stem Cells  Treatment for Ankylosing Spondylitis

This is a systemic rheumatic disease, meaning it affects the entire body. Approximately 90% of AS patients express the HLA-B27 genotype, meaning there is a strong genetic association. However, only 5% of individuals with the HLA-B27 genotype contract the disease. Tumor necrosis factor-alpha (TNF ?) and IL-1 are also implicated in ankylosing spondylitis. Auto antibodies specific for AS have not been identified. Antineutrophil cytoplasmic antibodies ANCA are associated with AS, but do not correlate with disease severity.The association of AS with HLA-B27 suggests the condition involves CD8 T cells, which interact with HLA-B.

Ankylosing Spondylitis treatment mumbai

Clinical Presentation:

  • Ankylosing Spondylitis begins with pain in the lower spine and sacroiliac joints with muscle spasm, often spreading to the upper back and neck and improved by exercise.
  • Chronic pain and stiffness in the lower part of the spine or sometimes the entire spine with pain referred to one or other buttock or the back of thigh from the sacroiliac joint.
  • Inflammation of the eye causing eye pain.
  • Fatigue and sometimes nausea.
  • Lung fibrosis.
  • Escatsia of the sacral nerve root sheaths may occur causing pain and swelling of large limb joints.

Now, such incurable diseases can be treated by Stem Cell Therapy. Where Stem cells are being taken from patient’s own bone marrow, Adipose derived fat stem cells, peripheral blood derived stem cells or Umbilical cord blood-derived & placenta-derived immune rejection free stem cells.

Stem cells are immature, unprogrammed, unspecialised precursor cells that can grow and change to make new cells and tissues. They can transform into about 250 types of specialised cells that make up a human being.

Ankylosing Spondylitis treatment India

In recent years, scientists have developed a new method for treating autoimmune disease like Ankylosing Spondylitis, with Mesenchymal stem cells implantation. It has several advantages:

  • The Mesenchymal stem cells can differentiate into immune cells, repair the immune system, some of them after implantation can repair and replace the damaged cells and tissue.
  • The Mesenchymal stem cells can secrete different kinds of cell factors which can withhold inflammatory mediators and cells, repair damaged joints and the synovium, and block the development of the disease effectively.
  • There is no risk of a rejection reaction, it is safe and effective and the most important thing is that the stem cells treatment has a very measurable, positive effect.

We hope in future by using the stem cell culture we can produce the artificial vertebrae and can treat the Ankylosing Spondylitis diseases.

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Regeneration of meniscus and hyaline cartilage in a arthitic knee treated with intra-articular implantation of autologous mesenchymal stem cells from bone marrow and or adipose derived (fat).

Regeneration of meniscus and hyaline cartilage in a arthitic knee treated with intra-articular implantation of autologous mesenchymal stem cells from bone marrow and or adipose derived (fat).

Stem Cell Treatment for Cartilage Stem Cell Treatment for Cartilage

Mesenchymal stem cells, or MSCs, are multipotent stromal cells that can differentiate into a variety of cell types,including: osteoblasts (bone cells), chondrocytes (cartilage cells), and adipocytes (fat cells).


Mesenchymal stem cells (MSCs) are adult autologous stem cells found in the bone marrow. However, mesenchymal stem cells can also be isolated from other tissues including cord blood, peripheral blood, fallopian tube, and fetal liver and lung. Mesenchymal stem cells are a distinct entity to the mesenchyme, embryonic connective tissue which is derived from the mesoderm and differentiates to form hematopoietic stem cells. Because of their multi-potent capabilities, mesenchymal stem cell (MSC) lineages have been used successfully in animal and human models to regenerate articular cartilage and bone.

Current treatment for cartilage damage in osteoarthritis focuses on surgical interventions such as arthroscopic debridement, microfracture, and cartilage grafting/transplant, total joint replacement. These procedures have more complications than hoped, are invasive, and often entail a prolonged recovery time. MSCs can be harvested from the iliac crest through Bone Marrow Aspiration, activated using the patient’s own growth factors from platelet’s (PRP), then successfully implanted to increase cartilage volume in an adult human knee.

We present a review highlighting the developments in cellular and regenerative medicine in the arena mesenchymal stem cell therapy by successful harvest and transplant of autologous mesenchymal stem cells into an adult human knee, hip, ankle, shoulder, fingers, facets in the spine and wrists that repairs the cartilage, ligaments & meniscal damage & lubricate the joint space.

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Applications of Mesenchymal stem cell-based therapies in Rheumatoid Arthritis.

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Ozone injections for herniated disc. Prolozone used for chronic back pain from disc damage.

Below you will find some technical information on the use of ozone injections into and round the spinal discs of the back to promote recovery from chronic back pain. Ozone is being widely used and accepted as an alternative treatment to surgery and may be much better option due to it’s less invasive approach. It is more widely used out side of the USA due to political power of the surgical and pharmacology influences that govern the education and exposure to medical professionals here. There are 2 approaches the direct and the indirect approach.

The direct approach is carried out under radioscopic control: the needle is

inserted in the centre of the pathologic intersomatic space just before direct

insufflation of the gas mixture.  An expert can do it in about 10

min. After a rest of 10-15 min, the patient can get up and often he/she is

amazed by the disappearance of the pain, as occurs after nucleoplasty. If

necessary, the application can be repeated a second time before changing the


Good results have been obtained after either intradiscal or intraforaminal

injection of a variable volume (3-15 ml) of gas at an O3 concentration of 27-

30 mcg/ml. Several thousand patients have been treated, with a success rate

of 54-86% ( Jucopilla et al., 2000; Bonetti et al., 2001; Fabris et al., 2001;

Petralia et al., 2001; Alexandre et al., 2002). An extensive study had been

performed in 600 patients, who had failed to respond to conservative

management (Andreula et al., 2003): 70.3% of the first half of patients,

treated only with ozone, showed a good outcome. This was further improved

(78.3%) in the remaining 300 patients, by combining ozone treatment with a

periganglionic injection of corticosteroid and anaesthetic. Unfortunately

controls (either oxygen or oxygen-corticosteroid-anaesthetic alone) were not

evaluated, probably, for ethical reasons. Nonetheless, from a scientic point

of view, it will be important to perform a randomized study to evaluate the

role of the needle, oxygen and so forth, which are probably relevant.

It remains unclear how ozone acts. One real possibility, previously

discussed at length (Bocci 1998a, 1999), is that ozone dissolves in the

interstitial water and reacts immediately, generating a cascade of ROS,

among which H2O2 and possibly the hydroxyl radical, which is most

reactive. The hydroxyl radical can react with carbohydrates and amino acids

composing proteoglycans and collagen type I and II, major components of

the degenerate nucleus pulposus, leading to its breakdown (McCord, 1974;

Curran et al., 1984; Hawkins and Davies, 1996; Bocci et al., 2001b;

Leonardi et al., 2001). These studies, as well as those performed on human

blood, have been carried out using the Electron Paramagnetic Resonance

(EPR) spin trapping technique (Ueno et al., 1998; Bocci et al., 2001b).

Consequently, reabsorption of hydrolytic products and water may lead to

progressive shrinkage and disappearance of the herniated material. Reduced

mechanical irritation decreases the sensitivity of nerve axons, but

nociceptors are also excited by endogenous algesic substances released

during perineural ischaemia or neural inflammation present in the spinal

ganglion and neural roots (Willis, 1995). Thus, more than the mechanical

compression as primum movens, it is the inflammatory reaction that

sustains chronic pain by releasing PLA2, several proteinases and

cytokines. The continued release of ROS, PGE2, serotonin, bradykinin,

cathepsins, IL-1, IL-6, substance P and TNF alpha causes oedema, possibly

demyelination and increased excitability of nociceptors (Fields, 1986).


Indeed, it has been observed that, in absence of inflammation, even a large

hernia can be painless. Moreover, the hernia may remain after an operation

(as seen radiographically), but the pain disappears once the inflammatory

disorder dies down. Interestingly, epidural injections of the antiinflammatory

methylprednisolone transitorily improve leg pain and sensory

deficits in patients with sciatica due to a herniated disc (Carette et al., 1997).

Table 7 intends to summarize the complex reparative process induced by

ozone used in substitution or in combination with orthodox remedies.

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Osteoarthritis and the use of Ozone Injections for regeneration of cartilage and ligaments explained. | Sarasota, Florida doctor uses ozone for arthritis

Wear and tear of joints and break down of cartilage called osteoarthritis. The

pathophysiology of these diseases is complex and characterized by

the softening and even distruction of the articular cartilage, with increased

matrix degradation due to collagenase and proteoglycanases. The enzymes

may be secreted by activated chondrocytes and monocytes, which release IL-

1 and TNF

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Using adipose derived stem cells in treatments for arthritis, muscle and tendon tears.

The above picture is a study done in Oct. 2012 showing the difference in doing nothing , PRP , ADSC (fat), and BMSC (bone marrow) on cartilage damage. They looked at 6 & 12 wk’s. This evidence supports the use of BMA and ADSC in moderate to severe arthritis for the regeneration of cartilage.

The above picture is a study done in Oct. 2012 showing the difference in doing nothing , PRP , ADSC (fat), and BMSC (bone marrow) on cartilage damage. They looked at 6 & 12 wk’s. This evidence supports the use of BMA and ADSC in moderate to severe arthritis for the regeneration of cartilage.

At Gecko Joint and Spine we use autologous adipose derived stem cells in our treatments. Adult Stem Cells (ASCs), by definition, are unspecialized or undifferentiated cells that not only retain their ability to divide mitotically while still maintaining their undifferentiated state but also, given the right conditions, have the ability to differentiate into different types of cells including cells of different germ-origin – an ability referred to as transdifferentiation or plasticity.1,2 In vitro, the conditions under which transdifferentiation occurs can be brought about by modifying the culture medium in which the cells are cultured. In vivo, the same changes are seen when the ASCs are transplanted into a tissue environment different to their own tissue-of origin. Though the exact mechanism of this transdifferentiation of ASCs is still under debate, this ability of ASCs along with their ability to self-renew is of great interest in the field of Regenerative Medicine as a therapeutic tool in being able to regenerate and replace dying, damaged or diseased tissue.

Clinically, however, there are a few criteria that ASCs need to fulfill before they can be viewed as a viable option in Regenerative Medicine. These are as follows:3

  1. Abundance in numbers (millions to billions of cells)
  2. Ease of harvest (through minimally invasive procedures)
  3. Ability to differentiate into multiple cell types (which can be regulated and reproduced in vitro)
  4. Safe to transplant to a different site of the autologous host or even an allogenic host.
  5. No conflict with current Good Manufacturing Principles (during procurement, culture or transplantation)

To find out more about autologous derived adipose stem cells and how they might work to regenerate your arthritis or muscle or tendon tear then you can call our office at (941) 330-8553 or email


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